Holloway, Kathryn V. and O'Gorman, Martin and Woods, Paul and Morton, James P. and Evans, Louise and Cable, Nigel T. and Goldspink, David F. and Burniston, Jatin G. (2009) Proteomic investigation of changes in human vastus lateralis muscle in response to interval-exercise training. Proteomics, 9 (22). pp. 5155-5174. ISSN 1615-9853
Full text not available from this repository. (Request a copy)Abstract
No previous study has used proteomics to investigate the effects of exercise training on human skeletal muscle. Five recreationally active men completed a 6-wk training programme involving three sessions per week, utilising six 1-min bouts at maximum oxygen uptake (V̇ O2max) interspersed with 4 min at 50% V̇ O2max. Vastus lateralis was biopsied at standardised times before and after the training intervention. Protein expression profiling was performed using differential analysis of 2-DE gels; complemented with quantitative analysis (iTRAQ) of tryptic peptides from 1-DE gel lane-segments using LC-MALDI MS/MS. Interval training increased average V̇ O2max (7%; p<0.001) and was associated with greater expression of mitochondrial components, including succinate dehydrogenase, trifunctional protein-α and ATP synthase α- and β-chains. 2-DE resolved 256 spots, and paired t-tests identified 20 significant differences in expression (false discovery rate <10%). Each differentially expressed gene product was present as multiple isoelectric species. Therefore, the differences in spot expression represent changes in post-transcriptional or post-translational processing. In particular, modulation of muscle creatine kinase and troponin T were prominent. Pro-Q Diamond staining revealed these changes in expression were associated with phosphorylated protein species, which provides novel information regarding muscle adaptation to interval training.
| Item Type: | Article |
|---|---|
| Keywords: | 2-DE; Animal proteomics;Exercise training; iTRAQ; LC-MALDI-TOF/TOF MS; Skeletal muscle |
| Subjects: | Q Science > QM Human anatomy |
| Faculty / Department: | Faculty of Human and Digital Sciences > School of Health and Sport Sciences |
| Depositing User: | Susan Murray |
| Date Deposited: | 06 Mar 2014 09:37 |
| Last Modified: | 06 Mar 2014 09:37 |
| URI: | https://hira.hope.ac.uk/id/eprint/299 |
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