Effects of Pulsed Electromagnetic Fields on Breast Cancer Cell Line MCF 7 Using Absorption Spectroscopy

Alcantara, DZ and Soliman, IJS and Pobre, RF and Naguib, RNG (2017) Effects of Pulsed Electromagnetic Fields on Breast Cancer Cell Line MCF 7 Using Absorption Spectroscopy. Anticancer Research, 37 (7). pp. 3453-3459. ISSN 0250-7005

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Abstract

We present an analysis of the effects of pulsed electromagnetic fields (PEMF) with 3.3 MHz carrier frequency and modulated by audio resonant frequencies on MCF-7 breast cancer cell line in vitro using absorption spectroscopy. This involves a fluorescence dye called PrestoBlue™ Cell Viability Reagent and a spectrophotometry to test the viability of MCF-7 breast cancer cells under different PEMF treatment conditions in terms of the cell’s absorption values. The DNA molecule of the MCF-7 breast cancer cells has an electric dipole property that renders it sensitive and reactive to applied electromagnetic fields. Resonant frequencies derived from four genes mutated in MCF-7 breast cancer cells [rapamycin-insensitive companion of mammalian target of rapamycin (RICTOR), peroxisome proliferator-activated receptor (PPARG), Nijmegen breakage syndrome 1 (NBN) and checkpoint kinase 2 (CHEK2)] were applied in generating square pulsed electromagnetic waves. Effects were monitored through measurement of absorption of the samples with PrestoBlue™, and the significance of the treatment was determined using the t-test. There was a significant effect on MCF-7 cells after treatment with PEMF at the resonant frequencies of the following genes for specific durations of exposure: RICTOR for 10 minutes, PPARG for 10 minutes, NBN for 15 minutes, and CHEK2 for 5 minutes.

Item Type:	Article
Keywords:	Breast cancer, gene mutation, pulsed electromagnetic fields, spectroscopy, PrestoBlueT™
Faculty / Department:	Faculty of Human and Digital Sciences > Mathematics and Computer Science
Depositing User:	Raouf Naguib
Date Deposited:	31 Jul 2017 14:09
Last Modified:	31 Jul 2017 14:09
URI:	https://hira.hope.ac.uk/id/eprint/2080

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