Pattern Formation by Staphylococcus epidermidis via Droplet Evaporation on Micropillars Arrays at a Surface

Sussary-Arce, A and Martin, A and Massey, A and Oknianska, A and Diaz-Fernandez, Y and Hernandez-Sánchez, J.F and Griffiths, E and Gardeniers, J.G.E and Snoeijer, J.H and Lohse, Detlef and Ravak, R (2016) Pattern Formation by Staphylococcus epidermidis via Droplet Evaporation on Micropillars Arrays at a Surface. Langmuir, 32 (28). pp. 7159-7169. ISSN 0743-7463

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Official URL: https://pubs.acs.org/doi/abs/10.1021/acs.langmuir....

Abstract

We evaluate the effect of epoxy surface structuring on the evaporation of water droplets containing Staphylococcus epidermidis (S. epidermidis). During evaporation, droplets with S. epidermidis cells yield to complex wetting patterns such as the zipping-wetting1−3 and the coffee-stain effects. Depending on the height of the microstructure, the wetting fronts propagate circularly or in a stepwise manner, leading to the formation of octagonal or square-shaped deposition patterns.4,5 We observed that the shape of the dried droplets has considerable influence on the local spatial distribution of S. epidermidis deposited between micropillars. These changes are attributed to an unexplored interplay between the zipping-wetting1 and the coffee-stain6 effects in polygonally shaped droplets containing S. epidermidis. Induced capillary flows during evaporation of S. epidermidis are modeled with polystyrene particles. Bacterial viability measurements for S. epidermidis show high viability of planktonic cells, but low biomass deposition on the microstructured surfaces. Our findings provide insights into design criteria for the development of microstructured surfaces on which bacterial propagation could be controlled, limiting the use of biocides.

Item Type: Article
Additional Information and Comments: This document is the unedited Author’s version of a Submitted Work that was subsequently accepted for publication in Langmuir, © American Chemical Society 2016, after peer review. To access the final edited and published work see https://pubs.acs.org/doi/abs/10.1021/acs.langmuir.6b01658
Faculty / Department: Faculty of Science > School of Health Sciences
Depositing User: Matthew Adams
Date Deposited: 22 Nov 2018 15:09
Last Modified: 22 Nov 2018 15:09
URI: http://hira.hope.ac.uk/id/eprint/2682

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