Bacterial Footprints in Elastic Pillared Microstructures

Susarrey-Arce, Arturo and Hernandez-Sánchez, Jose Federico and Marcello, Marco and Diaz-Fernandez, Yuri and Oknianska, Alina and Sorzabal-Bellido, Ioritz and Tigelaar, Roald and Lohse, Detlef and Gardeniers, Han and Snoeijer, Jaco and Martin, Alvaro and Raval, Rasmita (2018) Bacterial Footprints in Elastic Pillared Microstructures. ACS Applied Bio Materials, 1 (5). pp. 1294-1300. ISSN 2576-6422

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Official URL: https://pubs.acs.org/doi/10.1021/acsabm.8b00176

Abstract

Soft substrates decorated with micropillar arrays are known to be sensitive to deflection due to capillary action. In this work, we demonstrate that micropillared epoxy surfaces are sensitive to single drops of bacterial suspensions. The micropillars can show significant deformations upon evaporation, just as capillary action does in soft substrates. The phenomenon has been studied with five bacterial strains: S. epidermidis, L. sakei, P. aeruginosa, E. coli, and B. subtilis. The results reveal that only droplets containing motile microbes with flagella stimulate micropillar bending, which leads to significant distortions and pillar aggregations forming dimers, trimers, and higher order clusters. Such deformation is manifested in characteristic patterns that are left on the microarrayed surface following evaporation and can be easily identified even by the naked eye. Our findings could lay the ground for the design and fabrication of mechanically responsive substrates, sensitive to specific types of microorganisms.

Item Type: Article
Additional Information and Comments: This document is the unedited Author’s version of a Submitted Work that was subsequently accepted for publication in Applied Bio Materials, © 2018 American Chemical Society. after peer review. To access the final edited and published work see https://pubs.acs.org/doi/10.1021/acsabm.8b00176
Faculty / Department: Faculty of Science > School of Health Sciences
Depositing User: Matthew Adams
Date Deposited: 22 Nov 2018 14:35
Last Modified: 22 Nov 2018 14:35
URI: http://hira.hope.ac.uk/id/eprint/2681

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