Direct comparison of methionine restriction with leucine restriction on the metabolic health of C57BL/6J mice

Lees, Emma K. and Banks, Ruth and Cook, Chelsea and Hill, Sophie and Morrice, Nicola and Grant, Louise and Mody, Nimesh and Delibegovic, Mirela (2017) Direct comparison of methionine restriction with leucine restriction on the metabolic health of C57BL/6J mice. Scientific Reports, 7 (1). ISSN 2045-2322

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The effects of methionine restriction (MR) in rodents are well established; it leads to decreased body and fat mass, improved glucose homeostasis and extended lifespan, despite increased energy intake. Leucine restriction (LR) replicates some, but not all, of these effects of MR. To determine any discrepancies in metabolic effects between MR and LR, this study compared 8 weeks of MR (80% restriction), LR (80% restriction) and control diet in 10-month-old C57BL/6 male mice. Body composition, food intake and glucose homeostasis were measured throughout the study and biochemical analyses of white adipose tissue (WAT) and liver were performed. MR and LR decreased body and fat mass, increased food intake, elevated lipid cycling in WAT and improved whole body glucose metabolism and hepatic insulin sensitivity in comparison to the control diet. MR produced more substantial effects than LR on body mass and glucose homeostasis and reduced hepatic lipogenic gene expression, which was absent with the LR diet. This could be a result of amino acid-specific pathways in the liver responsible for FGF21 stimulation (causing varied levels of FGF21 induction) and Akt activation. In summary, LR is effective at improving metabolic health; however, MR produces stronger effects, suggesting they activate distinct signalling pathways.

Item Type: Article
Additional Information and Comments: This is the author's version of an article that was accepted for publication in Scientific Reports. The final version is available at
Faculty / Department: Faculty of Science > School of Health Sciences
Depositing User: Emma Lees
Date Deposited: 16 Feb 2018 11:49
Last Modified: 16 Feb 2018 11:49

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